Diagnosticum vi vi antigenic erythrocyte. Diagnosticum erythrocyte salmonella o
Registration certificate No. RZN 2016/3905 dated 04/04/2016
Purpose
The set of reagents "Diagnosticum erythrocyte salmonella Vi-antigen for RPHA" (ED-Vi) is intended for the detection of antibodies to the Vi-antigen of the causative agent of typhoid fever in human blood sera by the reaction of passive hemagglutination (RPHA).
Characteristics of the set
Operating principle
In the presence of antibodies to the causative agent of typhoid fever, hemagglutination of chicken erythrocytes sensitized with the Vi antigen is observed, which leads to the formation of an “umbrella” of settled erythrocytes at the bottom of the U-shaped wells of the plate. In the absence of antibodies to the causative agent of typhoid fever, settled red blood cells form a “dot”.
Set contents
| Reagent name | Description | Quantity in set |
|---|---|---|
| Diagnosticum erythrocyte salmonella Vi-antigen, dry 6% (EDS) | Formalized chicken erythrocytes sensitized with the S. typhi Vi antigen. Dry hygroscopic mass of brown color. After dissolution, the suspension is red-brown in color. | 1 fl., from 0.6 ml |
| Salmonella diagnostic serum adsorbed, Vi receptor, dry (diluted 1:20, (K+)) | Rabbit serum Salmonella adsorbed, Vi receptor, diluted 1:20. Dry hygroscopic porous mass of white color. After dissolution, it is a transparent yellowish or colorless liquid. | 1 fl., from 0.3 ml |
| Test sample diluent (RSD) | Transparent liquid of blue-violet color. | 1 fl., 10 ml |
| Phosphate buffer solution (PBS) | Transparent colorless liquid. | 1 fl., 10 ml |
| Single use polymer tablet for immunological reactions | Single-use polymer tablet for immunological reactions made of transparent colorless polystyrene. | 1 PC. |
Diagnostic characteristics
The diagnosticum must be agglutinated in the RPHA with diagnostic salmonella serum adsorbed, Vi receptor, dry (1:20 dilution), to the titer indicated on the serum label. The conditional level of diagnostic characteristics of blood serum from healthy people should be considered a serum dilution of no higher than 1:20. Analysis time is 3040 minutes. The kit is designed to test 42 blood sera in the screening option or 10 blood sera in the titration option.
Precautionary measures
The kit is intended for in vitro diagnostic use only. The substances included in the kit are inactivated and safe. When working with the kit, you should comply with SP 1.3.2322-08 and SanPiN 2.1.7.2790-10.
Additional equipment and materials
Equipment, materials, solutions:
- 1-channel pipette dispensers with variable dosing volume 5 - 40 µl; 40 - 200 µl; 200 - 1000 µl and 1000 - 5000 µl;
- 8- or 12-channel pipette dispensers with variable dosing volumes of 5 - 40 µl and 40 - 200 µl;
- distilled water (GOST 6709-72).
Analyzed samples
The blood serum samples under study are stored at a temperature of 2 to 8 °C for no more than 3 days from the moment of blood collection. Whey can be stored frozen at a temperature not exceeding minus 18 °C for no more than 1 year. Before use, samples are thawed at a temperature of 16 to 25 °C and mixed by shaking. Re-freezing is not allowed. Samples with bacterial growth and hemolysis should not be used. Before performing the reaction, the test sera are heated at 56 °C for 30 minutes.
Carrying out analysis
Preparation of control diagnostic serum (K+)
Prepare a working solution of diagnostic Salmonella serum adsorbed, Vi receptor, dry (1:20 dilution) from 0.3 ml (K+). To do this, add 0.3 ml of phosphate buffer solution (PBS) to the contents of the bottle with K+. The remaining amount of serum can be aliquoted and stored frozen at a temperature not exceeding minus 18 ° C for no more than 6 months.
Preparation of Salmonella erythrocyte diagnosticum (SED)
To prepare a working dilution of the Salmonella erythrocyte diagnosticum suspension, 0.6 ml of distilled water is added to the contents of the bottle with dry 6% SED and left to hydrate for 2 hours at a temperature of 16 to 25 °C. Then 2.4 ml of phosphate buffer solution (PBS) is added to the solution. The working solution is stored at a temperature of 2 to 8 °C for no more than 1 month. Freezing is not allowed.
Statement of RPGA during screening of blood serums
Blood sera for screening studies are diluted in the wells of the tablet as follows:
- preliminary dilutions of 1:20 are prepared in the first wells of the plate, first adding 190 μl of RIP solution into them, then 10 μl of the test sera. Each serum is added with a separate tip and carefully pipetted (the color of the solution in the wells after adding the serums should change from blue-violet to green);
- screening dilutions of 1:40 are prepared in the second wells by first adding 25 μl of PBS solution into them, and then 25 μl of pre-diluted sera and carefully pipetting.
Each time RPGA is performed, it is necessary to carry out a control determination of the K+ titer. To do this, add 50 μl of PBS solution to 8 wells in a long row. Then add 50 μl of K+ working solution (1:20) to the first well, carefully pipet it and transfer 50 μl to the next wells, obtaining 2-fold dilutions from 1:40 to 1:5120. 50 μl of PBS solution is added to another 4 wells to monitor EDS for the absence of spontaneous hemagglutination.
25 μl of SED is added to all wells of the plate with screening dilutions of the test sera (except for the first ones containing RIP) and controls. Stir the SED suspension in a bottle or bath before use! The tablet is thoroughly shaken and left at a temperature of 16 to 25 ° C for 30 - 40 minutes until the erythrocytes in the control completely settle.
Statement of RPGA during titration of test blood sera
Titration of the test sera and K+ working solution is carried out in short rows of the plate. Another short row is used to monitor the absence of spontaneous hemagglutination of EDS.
180 μl of RIP solution is added to the first wells of short rows for titration of the test serum. Add 50 μl of PBS solution to all other wells.
20 μl of test sera are added to the wells with the RIP solution (a dilution of 1:10 is obtained). Each serum is added with its own tip and carefully pipetted (the color of the solution in the wells should change from blue-violet to green). Then, 50 μl is transferred from the first wells to the next wells in the rows, obtaining twofold dilutions from 1:20 to 1:1280. At the end of the titration, solutions in a volume of 50 μl are removed from the last wells.
Each time RPGA is performed, it is necessary to carry out a control determination of the K+ titer. To do this, add 50 μl of PBS solution to 8 wells in a long row. Then add 50 μl of K+ working solution (1:20) to the first well, carefully pipet it and transfer 50 μl to the next wells, obtaining 2-fold dilutions from 1:40 to 1:5120.
To control the diagnosticum for the absence of spontaneous hemagglutination, 50 μl of PBS solution is added to all wells of a short row.
25 μl of SED is added to all wells (except for the first wells of each row for test sera containing RIP). Stir the SED suspension in a bottle or bath before use! The tablet is thoroughly shaken and left at a temperature of 16 to 25 ° C for 30 - 40 minutes until the erythrocytes in the control completely settle.
Accounting and interpretation of results
Accounting for results when screening blood serum
The results are taken into account on a conventional scale of four crosses. The serum titer is considered to be its dilution, which gives hemagglutination by at least 3 (+++) crosses.
- ++++ (4+) - agglutinated erythrocytes form an inverted “umbrella” at the bottom of the hole, its edges fall off;
- +++ (3+) - agglutinated erythrocytes form an inverted “umbrella” at the bottom of the hole, its edges are smooth;
- ++ (2+) - along with agglutinated erythrocytes, at the bottom of the well there is a sediment in the form of a small “ring” of non-agglutinated erythrocytes;
- + (1+) - most red blood cells are not agglutinated and settle in the form of a small “ring”;
- (-) - non-agglutinated red blood cells form a “dot” at the bottom of the well.
A positive result is considered to be hemagglutination of erythrocytes loaded with Vi-antigen by at least 3 crosses (+++).
The quality control of the diagnosticum is provided by 4 wells of the control row, into which only PBS and SED solution were added. There should be no spontaneous hemagglutination in these wells - the reaction is negative (-). Otherwise, the study should be repeated. If hemagglutination appears upon re-staging, the drug is not used.
Sera with a negative result should be considered not to contain antibodies to the Vi antigen with a diagnostic titer of 1:40 or lower.
Sera that give a positive result at a dilution of 1:40 should be retested with titration of the serum to establish its titer.
Accounting for results when titrating blood serum
The serum titer is considered to be its dilution, which gives hemagglutination of at least 3 (+++) crosses.
The quality control of the diagnosticum is provided by the wells of the row for controlling EDS. There should be no spontaneous hemagglutination in these wells - the reaction is negative (-). Otherwise, the study should be repeated. If hemagglutination appears upon re-staging, the drug is not used.
The Diagnosticum Salmonella VI-antigen reagent kit is intended for the detection in human blood serum of specific antibodies to the Salmonella typhus VI-antigen in a passive hemagglutination reaction (RPHA).
SET CHARACTERISTICS
2.1. Principle of the method.
The active principle of Diagnosticum Salmonella VI-antigen is the Vi-antigen fixed on the surface of red blood cells. When interacting with sera containing antibodies to the Vi-antigen, the phenomenon of erythrocyte agglutination is observed.
2.2. SET CONTENTS
| Reagents | Quantity |
| Diagnosticum erythrocyte salmonella Vi-antigenic liquid- is a 1% suspension of formalinized and sensitized Salmonella Typhoid sheep erythrocytes with B-antigen in a phosphate buffer solution (pH 7.2 + 0.2; concentration 0.06 mol/l). Homogeneous suspension of brown color without flakes; upon settling, 2 layers are formed: a dense brown sediment of erythrocytes and a transparent yellowish supernatant liquid | 1 bottle - 3 ml |
| Salmonella diagnostic serum adsorbed B receptor dry - homogeneous mass from white with a brownish tint to beige | 1 bottle – 0.1 ml |
| 1% suspension of formalinized, unsensitized sheep erythrocytes— homogeneous suspension of brown color without flakes; upon settling, 2 layers are formed: a dense brown sediment of erythrocytes and a transparent yellowish supernatant liquid | 1 bottle – |
| Solution for diluting serum and staging RPGA - 0.9% sodium chloride solution - transparent colorless liquid, pH from 6.5 to 7.5 | 2 bottles – 8 ml each |
| Single-use round-bottom plate for immunological reactions - consists of 8 rows, each of which includes 12 holes with a transparent, colorless, round bottom | 1 PC |
ANALYTICAL AND DIAGNOSTIC CHARACTERISTICS.
3.1. The diagnosticum must be agglutinated in the RPHA with the diagnostic Salmonella serum adsorbed by the dry B receptor in a dilution of no less than 1:160.
The conditional level of diagnostic characteristics of blood serum from healthy people should be considered a serum dilution of no higher than 1:20.
3.2. Analysis time: 2 hours.
3.3. The set contains 8 definitions.
PRECAUTIONARY MEASURES
When working with the kit, you should comply with the “Rules of design, safety precautions, industrial sanitation, anti-epidemic regime and personal hygiene when working in laboratories (departments, departments) of sanitary and epidemiological institutions of the USSR Ministry of Health (M., 1981).
The analyzed sera, as well as reagents in contact with them, should be considered as potentially infected, capable of long-term preservation or transmission of HIV, hepatitis virus or any other causative agent of viral infection - they should be handled with care:
- work with rubber gloves;
- when pipetting it is necessary to use automatic dispensers;
- upon completion of work, treat the analyzed sera and reagents and instruments in contact with them with a disinfectant solution
- Wipe equipment with 70% ethyl alcohol before and after use.
The analyzed sera must be inactivated at a temperature of 56 0 C for 30 minutes.
The diagnostic Salmonella serum adsorbed by the B receptor, included in the kit, is dry and inactivated.
Objective analysis results are guaranteed if the following conditions are met:
- All kit reagents should be stored at temperatures from 2 to 8 0 C;
- do not use expired reagents;
- do not use the kit reagents if there is no appropriate marking on their packaging;
- To perform RPHA, use reagents included only in this kit.
2.diagnosticum
4.from destroyed microbes (isolation of a specific Ag)
Erythrocyte HBs diagnosticum
2.diagnosticum
4. suspension of air-sheep treated with tannin and precipitated a\g HBs
5.to detect specific antibodies in blood serum
Diagnosticum gp120
2.diagnosticum
4.separate a\g ex. HIV
5. for detection of a\t HIV
Tetanus erythrocyte diagnosticum
2.diagnosticum
4. suspension of er.ram, treated with tannin and a\g of tetanus
5. to detect specific antibodies in blood serum (to tetanus
Cardiolipin antigen for microprecipitation
2.diagnosticum
4.extracted lipid fractions from the heart of a healthy bull
5.to detect specific antibodies in blood serum
Ultrasonic treponemal antigen
2.diagnosticum
4. of killed exc. syphilis
5.to detect specific antibodies in blood serum
Corpuscular tularemia diagnosticum
2.diagnosticum
4.from individual pathogen particles
5.to detect specific antibodies in blood serum
Dysentery diagnosticum
2.diagnosticum
4. suspension from killed animals
5.to detect specific antibodies in the patient’s blood serum
Erythrocyte diagnosticum from Shigella Sonne
2.diagnosticum
4.suspension of er.ram, treated with tonin
5.to detect specific antibodies in blood serum
Diagnosticum from Salmonella typhimurium
2.diagnosticum
4. of killed exc. salmonella
5.to detect specific antibodies in blood serum
Serums
Anti-gangrenous horse serum 5000 IU
2.serum
4.from the blood serum of hyperimmia horses with anatoxin of excitatory gas gangrene
6. parenterally, after a test with globulin 1:100
Normal human immunoglobulin
2.serum
4.from donor blood serum
5. form-e pass. specific purchased arts imm
6.parenteral
Precipitating anthrax serum
2.serum
4. from the blood serum of hyperimmunized animals a\g exc.sib. ulcers
6.precipitation reaction (RP)
Botulinum serum type – A 400 IU
2.serum
5.to detect a specific antigen in the test material
6.neutralization reaction (RN)
Anti-anthrax globulin
2.serum
4.from the blood serum of hyperimmunized animals and/or exc. anthrax
5. form-e pass. specific purchased arts imm
6.parenteral
Hemolytic serum
2.serum
4.from the serum of hyperimmunized animals with erythrocytes of animals of another type
5.to detect a specific antigen in the test material
Ogawa cholera agglutinating serum
2.serum
4.from blood serum of hyperimmunized animals
5.to detect a specific antigen in the test material
Luminescent tularemia serum
2.serum
4.from the blood serum of hyperimmunized animals a\g exc.tular.
5.to detect a specific antigen in the test material
Immunoglobulin against human tick-borne encephalitis
2.serum
4.from donor blood serum
5. form-e pass. specific purchased arts imm
6.parenteral
ESNO diagnostic serum
2.serum
4.from the blood serum of animals hyperimmunized with ESNO viruses
5.to detect a specific antigen in the test material
Human immunoglobulin against hepatitis B
2.serum
4.from the blood serum of a donor vaccinated against hepatitis B
5. form-e pass. specific purchased arts imm
6.parenteral
Peroxidase-labeled antiglobulin serum
2.serum
4.from blood serum of hyperimmunized animals
5.to detect a specific antigen in the test material (HIV)
Anti-rabies immunoglobulin
2.serum
4.from donor blood serum
5. form-e pass. specific purchased arts imm
Registration certificate No. RZN 2016/3905 dated 04/04/2016
Purpose
The set of reagents "Diagnosticum erythrocyte salmonella Vi-antigen for RPHA" (ED-Vi) is intended for the detection of antibodies to the Vi-antigen of the causative agent of typhoid fever in human blood sera by the reaction of passive hemagglutination (RPHA).
Characteristics of the set
Operating principle
In the presence of antibodies to the causative agent of typhoid fever, hemagglutination of chicken erythrocytes sensitized with the Vi antigen is observed, which leads to the formation of an “umbrella” of settled erythrocytes at the bottom of the U-shaped wells of the plate. In the absence of antibodies to the causative agent of typhoid fever, settled red blood cells form a “dot”.
Set contents
| Reagent name | Description | Quantity in set |
|---|---|---|
| Diagnosticum erythrocyte salmonella Vi-antigen, dry 6% (EDS) | Formalized chicken erythrocytes sensitized with the S. typhi Vi antigen. Dry hygroscopic mass of brown color. After dissolution, the suspension is red-brown in color. | 1 fl., from 0.6 ml |
| Salmonella diagnostic serum adsorbed, Vi receptor, dry (diluted 1:20, (K+)) | Rabbit serum Salmonella adsorbed, Vi receptor, diluted 1:20. Dry hygroscopic porous mass of white color. After dissolution, it is a transparent yellowish or colorless liquid. | 1 fl., from 0.3 ml |
| Test sample diluent (RSD) | Transparent liquid of blue-violet color. | 1 fl., 10 ml |
| Phosphate buffer solution (PBS) | Transparent colorless liquid. | 1 fl., 10 ml |
| Single use polymer tablet for immunological reactions | Single-use polymer tablet for immunological reactions made of transparent colorless polystyrene. | 1 PC. |
Diagnostic characteristics
The diagnosticum must be agglutinated in the RPHA with diagnostic salmonella serum adsorbed, Vi receptor, dry (1:20 dilution), to the titer indicated on the serum label. The conditional level of diagnostic characteristics of blood serum from healthy people should be considered a serum dilution of no higher than 1:20. Analysis time is 3040 minutes. The kit is designed to test 42 blood sera in the screening option or 10 blood sera in the titration option.
Precautionary measures
The kit is intended for in vitro diagnostic use only. The substances included in the kit are inactivated and safe. When working with the kit, you should comply with SP 1.3.2322-08 and SanPiN 2.1.7.2790-10.
Additional equipment and materials
Equipment, materials, solutions:
- 1-channel pipette dispensers with variable dosing volume 5 - 40 µl; 40 - 200 µl; 200 - 1000 µl and 1000 - 5000 µl;
- 8- or 12-channel pipette dispensers with variable dosing volumes of 5 - 40 µl and 40 - 200 µl;
- distilled water (GOST 6709-72).
Analyzed samples
The blood serum samples under study are stored at a temperature of 2 to 8 °C for no more than 3 days from the moment of blood collection. Whey can be stored frozen at a temperature not exceeding minus 18 °C for no more than 1 year. Before use, samples are thawed at a temperature of 16 to 25 °C and mixed by shaking. Re-freezing is not allowed. Samples with bacterial growth and hemolysis should not be used. Before performing the reaction, the test sera are heated at 56 °C for 30 minutes.
Carrying out analysis
Preparation of control diagnostic serum (K+)
Prepare a working solution of diagnostic Salmonella serum adsorbed, Vi receptor, dry (1:20 dilution) from 0.3 ml (K+). To do this, add 0.3 ml of phosphate buffer solution (PBS) to the contents of the bottle with K+. The remaining amount of serum can be aliquoted and stored frozen at a temperature not exceeding minus 18 ° C for no more than 6 months.
Preparation of Salmonella erythrocyte diagnosticum (SED)
To prepare a working dilution of the Salmonella erythrocyte diagnosticum suspension, 0.6 ml of distilled water is added to the contents of the bottle with dry 6% SED and left to hydrate for 2 hours at a temperature of 16 to 25 °C. Then 2.4 ml of phosphate buffer solution (PBS) is added to the solution. The working solution is stored at a temperature of 2 to 8 °C for no more than 1 month. Freezing is not allowed.
Statement of RPGA during screening of blood serums
Blood sera for screening studies are diluted in the wells of the tablet as follows:
- preliminary dilutions of 1:20 are prepared in the first wells of the plate, first adding 190 μl of RIP solution into them, then 10 μl of the test sera. Each serum is added with a separate tip and carefully pipetted (the color of the solution in the wells after adding the serums should change from blue-violet to green);
- screening dilutions of 1:40 are prepared in the second wells by first adding 25 μl of PBS solution into them, and then 25 μl of pre-diluted sera and carefully pipetting.
Each time RPGA is performed, it is necessary to carry out a control determination of the K+ titer. To do this, add 50 μl of PBS solution to 8 wells in a long row. Then add 50 μl of K+ working solution (1:20) to the first well, carefully pipet it and transfer 50 μl to the next wells, obtaining 2-fold dilutions from 1:40 to 1:5120. 50 μl of PBS solution is added to another 4 wells to monitor EDS for the absence of spontaneous hemagglutination.
25 μl of SED is added to all wells of the plate with screening dilutions of the test sera (except for the first ones containing RIP) and controls. Stir the SED suspension in a bottle or bath before use! The tablet is thoroughly shaken and left at a temperature of 16 to 25 ° C for 30 - 40 minutes until the erythrocytes in the control completely settle.
Statement of RPGA during titration of test blood sera
Titration of the test sera and K+ working solution is carried out in short rows of the plate. Another short row is used to monitor the absence of spontaneous hemagglutination of EDS.
180 μl of RIP solution is added to the first wells of short rows for titration of the test serum. Add 50 μl of PBS solution to all other wells.
20 μl of test sera are added to the wells with the RIP solution (a dilution of 1:10 is obtained). Each serum is added with its own tip and carefully pipetted (the color of the solution in the wells should change from blue-violet to green). Then, 50 μl is transferred from the first wells to the next wells in the rows, obtaining twofold dilutions from 1:20 to 1:1280. At the end of the titration, solutions in a volume of 50 μl are removed from the last wells.
Each time RPGA is performed, it is necessary to carry out a control determination of the K+ titer. To do this, add 50 μl of PBS solution to 8 wells in a long row. Then add 50 μl of K+ working solution (1:20) to the first well, carefully pipet it and transfer 50 μl to the next wells, obtaining 2-fold dilutions from 1:40 to 1:5120.
To control the diagnosticum for the absence of spontaneous hemagglutination, 50 μl of PBS solution is added to all wells of a short row.
25 μl of SED is added to all wells (except for the first wells of each row for test sera containing RIP). Stir the SED suspension in a bottle or bath before use! The tablet is thoroughly shaken and left at a temperature of 16 to 25 ° C for 30 - 40 minutes until the erythrocytes in the control completely settle.
Accounting and interpretation of results
Accounting for results when screening blood serum
The results are taken into account on a conventional scale of four crosses. The serum titer is considered to be its dilution, which gives hemagglutination by at least 3 (+++) crosses.
- ++++ (4+) - agglutinated erythrocytes form an inverted “umbrella” at the bottom of the hole, its edges fall off;
- +++ (3+) - agglutinated erythrocytes form an inverted “umbrella” at the bottom of the hole, its edges are smooth;
- ++ (2+) - along with agglutinated erythrocytes, at the bottom of the well there is a sediment in the form of a small “ring” of non-agglutinated erythrocytes;
- + (1+) - most red blood cells are not agglutinated and settle in the form of a small “ring”;
- (-) - non-agglutinated red blood cells form a “dot” at the bottom of the well.
A positive result is considered to be hemagglutination of erythrocytes loaded with Vi-antigen by at least 3 crosses (+++).
The quality control of the diagnosticum is provided by 4 wells of the control row, into which only PBS and SED solution were added. There should be no spontaneous hemagglutination in these wells - the reaction is negative (-). Otherwise, the study should be repeated. If hemagglutination appears upon re-staging, the drug is not used.
Sera with a negative result should be considered not to contain antibodies to the Vi antigen with a diagnostic titer of 1:40 or lower.
Sera that give a positive result at a dilution of 1:40 should be retested with titration of the serum to establish its titer.
Accounting for results when titrating blood serum
The serum titer is considered to be its dilution, which gives hemagglutination of at least 3 (+++) crosses.
The quality control of the diagnosticum is provided by the wells of the row for controlling EDS. There should be no spontaneous hemagglutination in these wells - the reaction is negative (-). Otherwise, the study should be repeated. If hemagglutination appears upon re-staging, the drug is not used.
The Diagnosticum Salmonella VI-antigen reagent kit is intended for the detection in human blood serum of specific antibodies to the Salmonella typhus VI-antigen in a passive hemagglutination reaction (RPHA).
SET CHARACTERISTICS
2.1. Principle of the method.
The active principle of Diagnosticum Salmonella VI-antigen is the Vi-antigen fixed on the surface of red blood cells. When interacting with sera containing antibodies to the Vi-antigen, the phenomenon of erythrocyte agglutination is observed.
2.2. SET CONTENTS
| Reagents | Quantity |
| Diagnosticum erythrocyte salmonella Vi-antigenic liquid- is a 1% suspension of formalinized and sensitized Salmonella Typhoid sheep erythrocytes with B-antigen in a phosphate buffer solution (pH 7.2 + 0.2; concentration 0.06 mol/l). Homogeneous suspension of brown color without flakes; upon settling, 2 layers are formed: a dense brown sediment of erythrocytes and a transparent yellowish supernatant liquid | 1 bottle - 6 ml |
| Salmonella diagnostic serum adsorbed B receptor dry - homogeneous mass from white with a brownish tint to beige | 1 bottle – 0.1 ml |
| 1% suspension of formalinized, unsensitized sheep erythrocytes— homogeneous suspension of brown color without flakes; upon settling, 2 layers are formed: a dense brown sediment of erythrocytes and a transparent yellowish supernatant liquid | 1 bottle – |
| Solution for diluting serum and staging RPGA - 0.9% sodium chloride solution - transparent colorless liquid, pH from 6.5 to 7.5 | 2 bottles – 8 ml each |
| Single-use round-bottom plate for immunological reactions - consists of 8 rows, each of which includes 12 holes with a transparent, colorless, round bottom | 1 PC |
ANALYTICAL AND DIAGNOSTIC CHARACTERISTICS.
3.1. The diagnosticum must be agglutinated in the RPHA with the diagnostic Salmonella serum adsorbed by the dry B receptor in a dilution of no less than 1:160.
The conditional level of diagnostic characteristics of blood serum from healthy people should be considered a serum dilution of no higher than 1:20.
3.2. Analysis time: 2 hours.
3.3. The set contains 8 definitions.
PRECAUTIONARY MEASURES
When working with the kit, you should comply with the “Rules of design, safety precautions, industrial sanitation, anti-epidemic regime and personal hygiene when working in laboratories (departments, departments) of sanitary and epidemiological institutions of the USSR Ministry of Health (M., 1981).
The analyzed sera, as well as reagents in contact with them, should be considered as potentially infected, capable of long-term preservation or transmission of HIV, hepatitis virus or any other causative agent of viral infection - they should be handled with care:
- work with rubber gloves;
- when pipetting it is necessary to use automatic dispensers;
- upon completion of work, treat the analyzed sera and reagents and instruments in contact with them with a disinfectant solution
- Wipe equipment with 70% ethyl alcohol before and after use.
The analyzed sera must be inactivated at a temperature of 56 0 C for 30 minutes.
The diagnostic Salmonella serum adsorbed by the B receptor, included in the kit, is dry and inactivated.
Objective analysis results are guaranteed if the following conditions are met:
- All kit reagents should be stored at temperatures from 2 to 8 0 C;
- do not use expired reagents;
- do not use the kit reagents if there is no appropriate marking on their packaging;
- To perform RPHA, use reagents included only in this kit.
Vi-Diagnosticum
Diagnosticum Vi
Vi-Antigenic
